Western blot (WB): | 1:500-2000 |
Figure 1. Western blot analysis of AFG3L2 using anti-AFG3L2 antibody (AZA9JRG9). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AFG3L2 antigen affinity purified polyclonal antibody (AZA9JRG9) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AFG3L2 at approximately 80-90 kDa. The expected band size for AFG3L2 is at 89 kDa.
Figure 1. Western blot analysis of AFG3L2 using anti-AFG3L2 antibody (AZA9JRG9). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AFG3L2 antigen affinity purified polyclonal antibody (AZA9JRG9) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AFG3L2 at approximately 80-90 kDa. The expected band size for AFG3L2 is at 89 kDa.