Anti-Zebrafish ATG5 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All IHC

AZF1RDC0

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. IHC analysis of ATG5 using anti-ATG5 antibody (AZF1RDC0).
ATG5 was detected in a paraffin-embedded section of zebrafish cerebellum tissue. The tissue section was incubated with rabbit anti-ATG5 Antibody (AZF1RDC0) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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产品简介>

产品名称

Anti-Zebrafish ATG5 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

APG5; APG5 LIKE; APG5L; Apoptosis specific protein; ASP; ATG5; Autophagy protein 5; hAPG5

产品类型

Polyclonal

检验物种

zebrafish

应用范围

IHC

基因名称

ATG5

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived zebrafish ATG5 recombinant protein (Position: S118-L155).

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is also known as APG5 or ASP, and this gene is mapped to 6q21. It is found that knockdown of ATG5 in hepatocytes increased triglyceride levels with oleate or a second endogenous stimulus for triglyceride formation. These hepatocytes with ATG5 knockdown also had increased lipid droplet number and size. ATG5 is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.

Uniprot ID

F1RDC0

Gene ID

494180

文献引用格式

Zebrafish ATG5 Antibody (Boster Biological Technology, Wuhan, China. Catalog#AZF1RDC0)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

Autophagy protein 5

蛋白名全称

Autophagy protein 5

实验方案>

实验步骤

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