Western blot (WB): | 1:500-2000 |
Figure 1. Western blot analysis of CCT2 using anti-CCT2 antibody (AZQ6PBW6). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish tissue lysates,
Lane 2: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CCT2 antigen affinity purified polyclonal antibody (AZQ6PBW6) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CCT2 at approximately 58 kDa. The expected band size for CCT2 is at 58 kDa.
Figure 1. Western blot analysis of CCT2 using anti-CCT2 antibody (AZQ6PBW6). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish tissue lysates,
Lane 2: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CCT2 antigen affinity purified polyclonal antibody (AZQ6PBW6) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CCT2 at approximately 58 kDa. The expected band size for CCT2 is at 58 kDa.