Anti-Zebrafish ABCE1 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC

AZQ6TNW3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of ABCE1 using anti-ABCE1 antibody (AZQ6TNW3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCE1 antigen affinity purified polyclonal antibody (AZQ6TNW3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ABCE1 at approximately 67 kDa. The expected band size for ABCE1 is at 67 kDa.
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Figure 2. IHC analysis of ABCE1 using anti-ABCE1 antibody (AZQ6TNW3).
ABCE1 was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-ABCE1 Antibody (AZQ6TNW3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 3. IHC analysis of ABCE1 using anti-ABCE1 antibody (AZQ6TNW3).
ABCE1 was detected in a paraffin-embedded section of zebrafish eye tissue. The tissue section was incubated with rabbit anti-ABCE1 Antibody (AZQ6TNW3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Zebrafish ABCE1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ABC38; ABCE1; HuHP68; OABP; Ribonuclease 4 inhibitor; RLI; RNase L inhibitor; RNASEL1; RNASELI; RNS4I

产品类型

Polyclonal

检验物种

zebrafish

应用范围

WB, IHC

基因名称

ABCE1

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived zebrafish ABCE1 recombinant protein (Position: Q141-D599).

计算分子量

67 kDa

实际分子量

67 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

ATP binding cassette E1 (ABCE1, also RNase L inhibitor) is an ATPase found in humans involved in viral assembly. It is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily. ABCE1 inhibits the action of ribonuclease L. Ribonuclease L normally binds to 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) and inhibits the interferon-regulated 2-5A/RNase L pathway, which is used by viruses. ABCE1 heterodimerize with ribonuclease L and prevents its interaction with 2-5A, antagonizing the anti-viral properties of ribonuclease L, and allow the virus to synthesize viral proteins. It has also been implicated to have an effect in tumorcell proliferation and antiapoptosis.

Uniprot ID

Q6TNW3

Gene ID

406324

文献引用格式

Zebrafish ABCE1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#AZQ6TNW3)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

ATP binding cassette subfamily E member 1

蛋白名全称

ATP-binding cassette sub-family E member 1

实验方案>

实验步骤

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