Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
Immunofluorescence (IF): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Western blot analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Myelin basic protein/MBP antigen affinity purified polyclonal antibody (BA0094) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Myelin basic protein/MBP at approximately 16-22 kDa. The expected band size for Myelin basic protein/MBP is at 33 kDa.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of human brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MBP using anti-MBP antibody (BA0094).
MBP was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MBP using anti-MBP antibody (BA0094).
MBP was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MBP using anti-MBP antibody (BA0094).
MBP was detected in a paraffin-embedded section of mouse hippocampus tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MBP using anti-MBP antibody (BA0094).
MBP was detected in a paraffin-embedded section of mouse cerebral cortex tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MBP using anti-MBP antibody (BA0094).
MBP was detected in a paraffin-embedded section of rat cerebellum tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MBP using anti-MBP antibody (BA0094).
MBP was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MBP using anti-MBP antibody (BA0094).
MBP was detected in a paraffin-embedded section of rat hippocampus tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MBP using anti-MBP antibody (BA0094).
MBP was detected in a paraffin-embedded section of rat cerebral cortex tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of MBP using anti-MBP antibody (BA0094) MBP was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-MBP Antibody (BA0094) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IF analysis of MBP using anti-MBP antibody (BA0094) MBP was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-MBP Antibody (BA0094) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IF analysis of MBP using anti-MBP antibody (BA0094) MBP was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-MBP Antibody (BA0094) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IF analysis of MBP using anti-MBP antibody (BA0094) MBP was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-MBP Antibody (BA0094) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IF analysis of MBP using anti-GFAP antibody (MA1045) and anti-MBP antibody (BA0094) MBP was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6 epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL mouse anti-GFAP Antibody (MA1045)and anti-MBP Antibody (BA0094) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) and Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Western blot analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Myelin basic protein/MBP antigen affinity purified polyclonal antibody (BA0094) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Myelin basic protein/MBP at approximately 16-22 kDa. The expected band size for Myelin basic protein/MBP is at 33 kDa.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of human brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MBP using anti-MBP antibody (BA0094).
MBP was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MBP using anti-MBP antibody (BA0094).
MBP was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MBP using anti-MBP antibody (BA0094).
MBP was detected in a paraffin-embedded section of mouse hippocampus tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MBP using anti-MBP antibody (BA0094).
MBP was detected in a paraffin-embedded section of mouse cerebral cortex tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MBP using anti-MBP antibody (BA0094).
MBP was detected in a paraffin-embedded section of rat cerebellum tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MBP using anti-MBP antibody (BA0094).
MBP was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MBP using anti-MBP antibody (BA0094).
MBP was detected in a paraffin-embedded section of rat hippocampus tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of MBP using anti-MBP antibody (BA0094).
MBP was detected in a paraffin-embedded section of rat cerebral cortex tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of MBP using anti-MBP antibody (BA0094) MBP was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-MBP Antibody (BA0094) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IF analysis of MBP using anti-MBP antibody (BA0094) MBP was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-MBP Antibody (BA0094) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IF analysis of MBP using anti-MBP antibody (BA0094) MBP was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-MBP Antibody (BA0094) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IF analysis of MBP using anti-MBP antibody (BA0094) MBP was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-MBP Antibody (BA0094) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IF analysis of MBP using anti-GFAP antibody (MA1045) and anti-MBP antibody (BA0094) MBP was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6 epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL mouse anti-GFAP Antibody (MA1045)and anti-MBP Antibody (BA0094) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) and Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.