Western blot analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Myelin basic protein/MBP antigA03957-Aen affinity purified polyclonal antibody (BA0094) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Myelin basic protein/MBP at approximately 15-22 kDa. The expected band size for Myelin basic protein/MBP is at 33 kDa.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of human brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of pig brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of pig brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) (Catalog # BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:100. HRP Conjugated Goat Anti-Rabbit IgG (BA1054) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Tyramide signal amplification was performed using TSA 570 reagent at 1:200 dilution at room temperature for 10 minutes. The section was counterstained with DAPI. Fluorescence signals were visualized using a fluorescence microscope with filter sets appropriate for TSA 570 and DAPI.
| Western blot (WB): | 1:1000-5000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Myelin basic protein/MBP antigA03957-Aen affinity purified polyclonal antibody (BA0094) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Myelin basic protein/MBP at approximately 15-22 kDa. The expected band size for Myelin basic protein/MBP is at 33 kDa.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of human brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of pig brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of pig brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) (Catalog # BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:100. HRP Conjugated Goat Anti-Rabbit IgG (BA1054) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Tyramide signal amplification was performed using TSA 570 reagent at 1:200 dilution at room temperature for 10 minutes. The section was counterstained with DAPI. Fluorescence signals were visualized using a fluorescence microscope with filter sets appropriate for TSA 570 and DAPI.
Western blot analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Myelin basic protein/MBP antigA03957-Aen affinity purified polyclonal antibody (BA0094) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Myelin basic protein/MBP at approximately 15-22 kDa. The expected band size for Myelin basic protein/MBP is at 33 kDa.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of human brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of pig brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of pig brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) (Catalog # BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
Myelin basic protein/MBP was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:100. HRP Conjugated Goat Anti-Rabbit IgG (BA1054) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Tyramide signal amplification was performed using TSA 570 reagent at 1:200 dilution at room temperature for 10 minutes. The section was counterstained with DAPI. Fluorescence signals were visualized using a fluorescence microscope with filter sets appropriate for TSA 570 and DAPI.
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