欢迎访问博士德生物官网! 服务热线:027-67845390 |
您当前的位置: 首页 > 产品中心 > 一抗 > 多克隆抗体

Anti-Myelin basic protein/MBP Antibody

Rabbit polyclonal antibody

说明书 一键复制产品信息

筛选器: All WB IHC IF

BA0094

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat brain tissue tissue lysates,
    Lane 2: mouse brain tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Myelin basic protein/MBP antigen affinity purified polyclonal antibody (BA0094) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Myelin basic protein/MBP at approximately 16-22 kDa. The expected band size for Myelin basic protein/MBP is at 33 kDa.

    all(15)
  • IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (BA0094).
    Myelin basic protein/MBP was detected in a paraffin-embedded section of human brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(15)
  • IHC analysis of MBP using anti-MBP antibody (BA0094).
    MBP was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(15)
  • IHC analysis of MBP using anti-MBP antibody (BA0094).
    MBP was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(15)
  • IHC analysis of MBP using anti-MBP antibody (BA0094).
    MBP was detected in a paraffin-embedded section of mouse hippocampus tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(15)
  • IHC analysis of MBP using anti-MBP antibody (BA0094).
    MBP was detected in a paraffin-embedded section of mouse cerebral cortex tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(15)
  • IHC analysis of MBP using anti-MBP antibody (BA0094).
    MBP was detected in a paraffin-embedded section of rat cerebellum tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(15)
  • IHC analysis of MBP using anti-MBP antibody (BA0094).
    MBP was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(15)
  • IHC analysis of MBP using anti-MBP antibody (BA0094).
    MBP was detected in a paraffin-embedded section of rat hippocampus tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(15)
  • IHC analysis of MBP using anti-MBP antibody (BA0094).
    MBP was detected in a paraffin-embedded section of rat cerebral cortex tissue. The tissue section was incubated with rabbit anti-Myelin basic protein/MBP Antibody (BA0094) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(15)
  • IF analysis of MBP using anti-MBP antibody (BA0094) MBP was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-MBP Antibody (BA0094) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

    all(15)
  • IF analysis of MBP using anti-MBP antibody (BA0094) MBP was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-MBP Antibody (BA0094) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

    all(15)
  • IF analysis of MBP using anti-MBP antibody (BA0094) MBP was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-MBP Antibody (BA0094) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

    all(15)
  • IF analysis of MBP using anti-MBP antibody (BA0094) MBP was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-MBP Antibody (BA0094) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

    all(15)
  • IF analysis of MBP using anti-GFAP antibody (MA1045) and anti-MBP antibody (BA0094) MBP was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6 epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL mouse anti-GFAP Antibody (MA1045)and anti-MBP Antibody (BA0094) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) and Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

    all(15)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称
Anti-Myelin basic protein/MBP Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
MBP; Myelin A1 protein; Myelin basic protein
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, IF
基因名称
MBP
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the C-terminus of human Myelin Basic Protein, identical to the related rat and mouse sequences.
计算分子量
33 kDa
实际分子量
16-22 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Myelin basic protein(MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34 kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis.
Uniprot ID
P02686  
RRID
文献引用格式
Myelin basic protein/MBP Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0094)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
myelin basic protein
蛋白名全称
Myelin basic protein(MBP)
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Immunofluorescence (IF):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

实验方案>

实验步骤

引用文献(0)>

    相关产品>

    成功添加到购物车

    查看购物车 继续购物

    Anti-Myelin basic protein/MBP Antibody

    筛选器: All WB IHC IF

    亮暗模式切换
    X
    亮暗模式切换
    X

    在线
    客服

    在线客服
    服务时间:08:30 - 17:30

    点击下列客服在线咨询:

    服务热线

    服务
    热线

    客服服务热线

    027-67845390
    客服服务热线

    扫码

    扫码
    咨询

    微信二维码 微信扫一扫立即咨询
    返回顶部 顶部