Anti-TNF Alpha/TNFA Antibody

筛选器： All WB IHC FCM

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  Figure 1. Western blot analysis of anti- TNFA/TNF Alpha antibody (BA0131). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human U-937 whole cell lysates,
  Lane 2: rat spleen tissue lysates,
  Lane 3: rat C6 whole cell lysates,
  Lane 4: mouse spleen tissue lysates.
  Use rabbit anti- TNFA/TNF Alpha 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for TNFA/TNF Alpha at approximately 26KD. The expected band size for TNFA/TNF Alpha is at 26KD.

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  Figure 2. IHC analysis of TNF Alpha/TNFA using anti-TNF Alpha/TNFA antibody (BA0131).
  TNF Alpha/TNFA was detected in a paraffin-embedded section of human B lymphocytic tumor tissue. The tissue section was incubated with rabbit anti-TNF Alpha/TNFA Antibody (BA0131) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. Flow Cytometry analysis of Caco-2 cells using anti-TNF Alpha/TNFA antibody (BA0131).
  Overlay histogram showing Caco-2 cells stained with BA0131 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TNF Alpha/TNFA Antibody (BA0131) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Figure 1. Western blot analysis of anti- TNFA/TNF Alpha antibody (BA0131). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human U-937 whole cell lysates,
  Lane 2: rat spleen tissue lysates,
  Lane 3: rat C6 whole cell lysates,
  Lane 4: mouse spleen tissue lysates.
  Use rabbit anti- TNFA/TNF Alpha 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for TNFA/TNF Alpha at approximately 26KD. The expected band size for TNFA/TNF Alpha is at 26KD.

• 

  Figure 2. IHC analysis of TNF Alpha/TNFA using anti-TNF Alpha/TNFA antibody (BA0131).
  TNF Alpha/TNFA was detected in a paraffin-embedded section of human B lymphocytic tumor tissue. The tissue section was incubated with rabbit anti-TNF Alpha/TNFA Antibody (BA0131) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

• 

  Figure 3. Flow Cytometry analysis of Caco-2 cells using anti-TNF Alpha/TNFA antibody (BA0131).
  Overlay histogram showing Caco-2 cells stained with BA0131 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TNF Alpha/TNFA Antibody (BA0131) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.