Anti-Collagen Type II/COL2A1 Antibody

Rabbit polyclonal antibody

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筛选器： All IHC WB

BA0533

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. IHC analysis of Collagen Type II/COL2A1 using anti-Collagen Type II/COL2A1 antibody (BA0533).
Collagen Type II/COL2A1 was detected in a paraffin-embedded section of rat trachea tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Collagen Type II/COL2A1 Antibody (BA0533) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
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Figure 2. Western blot analysis of Collagen Type II/COL2A1 using anti-Collagen Type II/COL2A1 antibody (BA0533). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human COLO320 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Collagen Type II/COL2A1 antigen affinity purified polyclonal antibody (BA0533) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Collagen Type II/COL2A1 at approximately 180-190 kDa. The expected band size for Collagen Type II/COL2A1 is at 142 kDa.
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产品简介>

产品名称

Anti-Collagen Type II/COL2A1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Alpha 1 type II collagen; ANFH; AOM; COL11A3; COL2A1; Col2α1; Collagen alpha 1(II) chain; Collagen Type II; SEDC

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

IHC, WB

基因名称

COL2A1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen II, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

计算分子量

142 kDa

实际分子量

180-190 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Collagen, type II, alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocvtes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

Uniprot ID

P02458

文献引用格式

Collagen Type II/COL2A1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0533)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Collagen alpha-1(II) chain

蛋白名全称

Collagen alpha-1(II) chain

实验方案>

实验步骤

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