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Anti-MMP1 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

BA0568

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of MMP1 using anti-MMP1 antibody (BA0568). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human CEM whole cell lysates,
    Lane 3: human HT1080 whole cell lysates,
    Lane 4: human SMMC whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MMP1 antigen affinity purified polyclonal antibody (BA0568) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MMP1 at approximately 54 kDa. The expected band size for MMP1 is at 54 kDa.

    all(3)
  • IHC analysis of MMP1 using anti-MMP1 antibody (BA0568) .
    MMP1 was detected in a paraffin-embedded section of human cervical non-keratinized cervical squamous cell carcinoma tissue. The tissue section was incubated with rabbit anti-MMP1 Antibody (BA0568) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(3)
  • IHC analysis of MMP1 using anti-MMP1 antibody (BA0568) .
    MMP1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-MMP1 Antibody (BA0568) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(3)

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产品名称
Anti-MMP1 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CLG; CLGN; Fibroblast collagenase; Interstitial collagenase; Matrix metalloproteinase 1; MMP 1; MMP1
产品类型
Polyclonal
检验物种
human
应用范围
WB, IHC
基因名称
MMP1
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence in the middle region of human MMP1
计算分子量
54 kDa
实际分子量
54 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Matrix metalloproteinase-1 (MMP-1), also known as interstitial collagenase and fibroblast collagenase, is an enzyme that in humans is encoded by the MMP1 gene. MMP-1 was the first vertebrate collagenase both purified to homogeneity as a protein, and cloned as a cDNA. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. It is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.
Uniprot ID
P03956  
文献引用格式
MMP1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0568)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0001-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
matrix metallopeptidase 1
蛋白名全称
Interstitial collagenase
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-MMP1 Antibody

    筛选器: All WB IHC

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