Anti-COX-1/Cyclooxygenase-1/PTGS1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA0608

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of COX-1/Cyclooxygenase-1/PTGS1 using anti-COX-1/Cyclooxygenase-1/PTGS1 antibody (BA0608). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta liver tissue lysates,
Lane 2: A431 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-COX-1/Cyclooxygenase-1/PTGS1 antigen affinity purified polyclonal antibody (BA0608) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for COX-1/Cyclooxygenase-1/PTGS1 at approximately 70 kDa. The expected band size for COX-1/Cyclooxygenase-1/PTGS1 is at 69 kDa.
all(2)
IHC analysis of COX-1/Cyclooxygenase-1/PTGS1 using anti-COX-1/Cyclooxygenase-1/PTGS1 antibody (BA0608).
COX-1/Cyclooxygenase-1/PTGS1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-COX-1/Cyclooxygenase-1/PTGS1 Antibody (BA0608) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-COX-1/Cyclooxygenase-1/PTGS1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

COX1; COX-1; COX-1/Cyclooxygenase-1; COX3; Cyclooxygenase 1; PCOX1; PGG/HS; PGH synthase 1; PGHS 1; PGHS1; PHS 1; PHS1; Prostaglandin G/H synthase 1; Prostaglandin H2 synthase 1; PTGHS; PTGS1

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

PTGS1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human COX1, different from the related rat and mouse sequences by two amino acids.

计算分子量

69 kDa

实际分子量

70 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Cyclooxygenase 1(COX1), also known as Prostaglandin-endoperoxide synthase(PTGS1) or mitochondrial cytochrome c oxidase subunit 1, is the key enzyme in prostaglandin biosynthesis. The gene was approximately 40 kb long, with 11 protein-coding exons. There were 599 amino acid residues with a calculated molecular mass of approximately 68 kD. By analysis of a human/hamster somatic hybrid DNA panel, Funk et al.(1991) demonstrated that the PTGS1 gene maps to chromosome 9. Human prostaglandin endoperoxide synthase exhibited 91% amino acid identity with the sheep enzyme. Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration.

Uniprot ID

P23219

文献引用格式

COX-1/Cyclooxygenase-1/PTGS1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0608)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

prostaglandin-endoperoxide synthase 1(prostaglandin G/H synthase and cyclooxygenase)

蛋白名全称

Prostaglandin G/H synthase 1

实验方案>

实验步骤

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