Anti-Cyclin D1/CCND1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB FCM

BA0770-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Cyclin D1/CCND1 using anti-Cyclin D1/CCND1 antibody (BA0770-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Testis tissue lysates,
Lane 2: Human Placenta tissue lysates,
Lane 3: Rat Brain tissue lysates,
Lane 4: MCF-7 whole cell lysates,
Lane 5: COLO320 whole cell lysates,
Lane 6: SW620 whole cell lysates,
Lane 7: MM231 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cyclin D1/CCND1 antigen affinity purified polyclonal antibody (BA0770-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cyclin D1/CCND1 at approximately 34 kDa. The expected band size for Cyclin D1/CCND1 is at 34 kDa.
all(2)
Flow Cytometry analysis of U-87MG cells using anti- Cyclin D1 antibody (BA0770-2).
Overlay histogram showing U-87MG cells stained with BA0770-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyclin D1 Antibody (BA0770-2, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Cyclin D1/CCND1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

B cell lymphoma 1 protein; CCND1; cyclin D; Cyclin D1; D11S287E; G1/S specific cyclin D1; PRAD1; PRAD1 oncogene; U21B31

产品类型

Polyclonal

检验物种

human, rat

应用范围

WB, FCM

基因名称

CCND1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human Cyclin D1, different from the related mouse and rat sequences by two amino acids.

计算分子量

34 kDa

实际分子量

34 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Cyclin D1, also known as CCND1, is a human gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1-S phase of the cell cycle. Amplification or overexpression of cyclin D1 plays pivotal roles in the development of a subset of human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer. The cyclin D1 gene is overexpressed in human breast cancers and is required for oncogene-induced tumorigenesis. Brisken et al. (2003) found that prolactin (PRL; 176760) induced IGF2 (147470) mRNA and IGF2 induced cyclin D1 protein expression in mouse mammary epithelial cultures. And they also concluded that IGF2 is a mediator of prolactin-induced alveologenesis and that prolactin, IGF2, and cyclin D1 are components of a developmental pathway in mammary gland.

Uniprot ID

P24385

文献引用格式

Cyclin D1/CCND1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0770-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cyclin D1

蛋白名全称

G1/S-specific cyclin-D1

实验方案>

实验步骤

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