Western blot (WB): | 1:500-2000 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Western blot analysis of NGF beta/NGF using anti-NGF beta/NGF antibody (BA10611).
Lane 1: recombinant human NGFB protein 10ng,
Lane 2: recombinant human NGFB protein 5ng,
Lane 3: recombinant human NGFB protein 2.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NGF beta/NGF antigen affinity purified polyclonal antibody (BA10611) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NGF beta/NGF at approximately 14,27-35 kDa.
Western blot analysis of NGF beta/NGF using anti-NGF beta/NGF antibody (BA10611).
Lane 1: recombinant human NGFB protein 10ng,
Lane 2: recombinant human NGFB protein 5ng,
Lane 3: recombinant human NGFB protein 2.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NGF beta/NGF antigen affinity purified polyclonal antibody (BA10611) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NGF beta/NGF at approximately 14,27-35 kDa.