产品名称
HRP Conjugated Protein A
产品形态
Concentrated, Liquid
成分
1 mg/ml of HRP conjugated specific antibody; 0.01M PBS, 50% glycerol.
特异性
This peroxidase conjugate(HRP) conjugated Protein A. Human IgG1, IgG2 and IgG4 bind strongly, while IgG3 does not bind. Thereare also many instances in which monoclonal antibodies do not bind to Protein A, especially the majority of rat immunoglobulinsand mouse IgG1.
应用注释
Diluted by neutral PBS or TBS.Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
文献引用格式
HRP Conjugated Protein A (Boster Biological Technology, Wuhan, China. Catalog#BA1080)
建议工作浓度
Western Blotting: 1:3000-10000 (ECL)
Western Blotting: 1:300-1500 (DAB detection)
ELISA: 1:2000-10000 (TMB detection)
Optimal working dilutions must be determined by end users.
保存条件
At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
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