Anti-ABCA1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

BA1541-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ABCA1 using anti-ABCA1 antibody (BA1541-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Human HT1080 tissue lysates,
Lane 2: Human HELA tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCA1 antigen affinity purified polyclonal antibody (BA1541-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ABCA1 at approximately 254 kDa. The expected band size for ABCA1 is at 254 kDa.
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产品简介>

产品名称

Anti-ABCA1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ABC-1; ABC1; CERP; FLJ14958; HDLDT1; MGC164864; MGC165011; TGD; ABCA1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

ABCA1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human ABCA1 identical to the related mouse sequence.

计算分子量

254 kDa

实际分子量

254 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

ABCA1 (ATP-binding cassette, sub-family A (ABC1), member 1), also known as ABC1, the cholesterol efflux regulatory protein (CERP) is a protein which in humans is encoded by the ABCA1 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes contain 2,261 amino acids. Dot blot analysis of 50 tissues revealed ubiquitous expression of ABCA1 mRNA, with highest expression in placenta, liver, lung, adrenal glands, and all fetal tissues examined, and lowest expression in kidney, pancreas, pituitary, mammary gland, and bone marrow. This protein is a member of the ABCA subfamily. Members of the ABCA subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesterolefflux pump in the cellular lipid removal pathway. Using human ABCA1 expressed in the membrane fraction of sf9 insect cells, Szakacs et al. found specific, Mg(2+)-dependent ATP binding and low basal ATPase activity. Addition of potential lipid substrates or lipid acceptors did not modify the ATPase activity or nucleotide occlusion by ABCA1. Szakacs et al. speculated that ABCA1 may be a regulatory protein or may require other protein partners for full activation.

Uniprot ID

O95477

文献引用格式

ABCA1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1541-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

ATP-binding cassette, sub-family A (ABC1), member 1

蛋白名全称

ATP-binding cassette sub-family A member 1

实验方案>

实验步骤

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