Anti-Connexin-32/GJB1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA1592

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of Connexin-32/GJB1 using anti-Connexin-32/GJB1 antibody (BA1592). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Cardiac Muscle tissue lysates,
Lane 2: Rat Cardiac Muscle tissue lysates,
Lane 3: Rat Skeletal Muscle tissue lysates,
Lane 4: Rat Brain tissue lysates,
Lane 5: MCF-7 whole cell lysates,
Lane 6: HELA whole cell lysates,
Lane 7: SMMC whole cell lysates,
Lane 8: JURKAT whole cell lysates,
Lane 9: COLO320 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Connexin-32/GJB1 antigen affinity purified polyclonal antibody (BA1592) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Connexin-32/GJB1 at approximately 32 kDa. The expected band size for Connexin-32/GJB1 is at 32 kDa.
all(2)
Figure 2. IHC analysis of Connexin-32/GJB1 using anti-Connexin-32/GJB1 antibody (BA1592).
Connexin-32/GJB1 was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-Connexin-32/GJB1 Antibody (BA1592) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(2)

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产品名称

Anti-Connexin-32/GJB1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CMTX; CMTX1; Connexin-32; CX32; Gap junction beta 1 protein; GJB1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

GJB1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human Connexin 32/GJB1, identical to the related mouse and rat sequences.

计算分子量

32 kDa

实际分子量

32 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Gap junction beta-1 protein (GJB1), also known as connexin 32 (Cx32) is a transmembrane protein that in humans is encoded by the GJB1 gene. This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Uniprot ID

P08034

文献引用格式

Connexin-32/GJB1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1592)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

gap junction protein, beta 1, 32kDa

蛋白名全称

Gap junction beta-1 protein

实验方案>

实验步骤

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