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Anti-Integrin Alpha 4/ITGA4 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

BA1640

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of Integrin Alpha 4/ITGA4 using anti-Integrin Alpha 4/ITGA4 antibody (BA1640). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Jurkat whole cell lysates,
    Lane 2: human Hela whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Integrin Alpha 4/ITGA4 antigen affinity purified polyclonal antibody (BA1640) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Integrin Alpha 4/ITGA4 at approximately 115,150-220 kDa. The expected band size for Integrin Alpha 4/ITGA4 is at 115 kDa.

    all(2)
  • IHC analysis of Integrin Alpha 4/ITGA4 using anti-Integrin Alpha 4/ITGA4 antibody (BA1640).
    Integrin Alpha 4/ITGA4 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-Integrin Alpha 4/ITGA4 Antibody (BA1640) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(2)

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产品名称
Anti-Integrin Alpha 4/ITGA4 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CD49D; IA4; Integrin alpha 4; Integrin alpha IV; ITGA4; VLA 4 subunit alpha
产品类型
Polyclonal
检验物种
human, mouse, rat, rabbit
应用范围
WB, IHC
基因名称
ITGA4
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin alpha 4, different from the related mouse sequence by one amino acid.
计算分子量
115 kDa
实际分子量
115,150-220 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
ITGA4/Integrin, alpha4 is an integrin alpha subunit. It makes up half of the alpha4 beta1 lymphocyte homing receptor.The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain. ITGA4 was also mapped to 2q31-q32 by fluorescence in situ hybridization.
Uniprot ID
P13612  
文献引用格式
Integrin Alpha 4/ITGA4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1640)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
integrin, alpha 4(antigen CD49D, alpha 4 subunit of VLA-4 receptor)
蛋白名全称
Integrin alpha-4
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-Integrin Alpha 4/ITGA4 Antibody

    筛选器: All WB IHC

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