Anti-NPHS2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA1688

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of NPHS2 using anti-NPHS2 antibody (BA1688). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: 293T whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NPHS2 antigen affinity purified polyclonal antibody (BA1688) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NPHS2 at approximately 42 kDa. The expected band size for NPHS2 is at 42 kDa.
all(3)
Figure 2. IHC analysis of NPHS2 using anti-NPHS2 antibody (BA1688).
NPHS2 was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-NPHS2 Antibody (BA1688) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
all(3)
Figure 3. IHC analysis of NPHS2 using anti-NPHS2 antibody (BA1688).
NPHS2 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-NPHS2 Antibody (BA1688) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
all(3)

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产品简介>

产品名称

Anti-NPHS2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

NPHS2; PDCN; Podocin; SRN1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

NPHS2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human NPHS2, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

计算分子量

42 kDa

实际分子量

42 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Podocin(PDCN) is a protein which lines the podocytes and assists in maintaining the barrier at the glomerular basement membrane. NPHS2 is a causative gene for Familial idiopathic nephrotic syndromes, which represents a heterogeneous group of kidney disorders, and include autosomal recessive steroid-resistant nephrotic syndrome, which is characterized by early childhood onset of proteinuria, rapid progression to end-stage renal disease and focal segmental glomerulosclerosis. By positional cloning, NPHS2 was mapped to 1q25-31. It is almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli, and encodes a new integral membrane protein, podocin, belonging to the stomatin protein family. Boute et al.(2000) found ten different NPHS2 mutations, comprising nonsense, frameshift and missense mutations, to segregate with the disease, demonstrating a crucial role for podocin in the function of the glomerular filtration barrier.

Uniprot ID

Q9NP85

文献引用格式

NPHS2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1688)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

nephrosis 2, idiopathic, steroid-resistant(podocin)

蛋白名全称

Podocin

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实验步骤

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