Anti-MMP8 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA2201

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of MMP8 using anti-MMP8 antibody (BA2201). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates,
Lane 2: rat RH-35 whole cell lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MMP8 antigen affinity purified polyclonal antibody (BA2201) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MMP8 at approximately 53 kDa. The expected band size for MMP8 is at 53 kDa.
all(3)
IHC analysis of MMP8 using anti-MMP8 antibody (BA2201) .
MMP8 was detected in a paraffin-embedded section of mouse spleen tissue. The tissue section was incubated with rabbit anti-MMP8 Antibody (BA2201) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(3)
IHC analysis of MMP8 using anti-MMP8 antibody (BA2201) .
MMP8 was detected in a paraffin-embedded section of rat spleen tissue. The tissue section was incubated with rabbit anti-MMP8 Antibody (BA2201) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(3)

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产品简介>

产品名称

Anti-MMP8 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CLG1; HNC; Matrix metalloproteinase 8; MMP 8; MMP8; Neutrophil collagenase; PMNL CL; PMNL collagenase

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC

基因名称

MMP8

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human MMP8, different from the related rat and mouse sequences by three amino acids.

计算分子量

53 kDa

实际分子量

53 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

MMP8(Matrix metalloproteinase 8) is a member of the family of matrix metalloproteinases. It is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8 was mapped to 11q21-q22. MMP8 is an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The enzyme exhibits 58% homology to human fibroblast collagenase and has the same domain structure. It consists of a 20-residue signal peptide, and an 80-residue propeptide that is lost on autolytic activation by cleavage of an M-L bond. MMP8 was found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Matrix metalloproteinases(MMPs) have fundamental roles in tumor progression, but most clinical trials with MMP inhibitors have not shown improvements in individuals with cancer. MMP8 has a paradoxical protective role in cancer and provides a genetic model to evaluate the molecular basis of gender differences in cancer susceptibility.

Uniprot ID

P22894

文献引用格式

MMP8 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA2201)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

matrix metallopeptidase 8(neutrophil collagenase)

蛋白名全称

Neutrophil collagenase

实验方案>

实验步骤

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