Anti-TRPV3 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

BA2875

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of TRPV3 using anti-TRPV3 antibody (BA2875). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: A549 whole cell lysates,
Lane 3: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TRPV3 antigen affinity purified polyclonal antibody (BA2875) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TRPV3 at approximately 100 kDa. The expected band size for TRPV3 is at 91 kDa.
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产品简介>

产品名称

Anti-TRPV3 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

TRPV3; Vanilloid receptor like 3; VRL 3; VRL3

产品类型

Polyclonal

检验物种

human

应用范围

基因名称

TRPV3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human TRPV3, different from the related rat and mouse sequences by four amino acids.

计算分子量

91 kDa

实际分子量

100 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

TRPV3(Transient Receptor Potential Cation Channel Subfamily V Member 3), also known as VRL3, is a human gene encoding the protein of the same name. The TRPV3 protein belongs to a family of nonselective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. Peier et al.(2002) localized the TRPV3 gene to a BAC clone mapped to chromosome 17p13. They mapped the mouse gene to chromosome 11B4. Peier et al.(2002) stably expressed mouse Trpv3 in Chinese hamster ovary cells and assayed electrophysiologic activity by whole cell voltage-clamp techniques. They determined that Trpv3 is a cation-permeable channel activated by warm and hot temperatures. Xu et al.(2002) showed that increasing temperature from approximately 22 to 40 degrees Celsius in mammalian cells transfected with human TRPV3 elevated intracellular calcium by activating a nonselective cationic conductance.

Uniprot ID

Q8NET8

文献引用格式

TRPV3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA2875)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

transient receptor potential cation channel, subfamily V, member 3

蛋白名全称

Transient receptor potential cation channel subfamily V member 3

实验方案>

实验步骤

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