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Anti-SLC16A4 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

BA3245

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of SLC16A4 using anti-SLC16A4 antibody (BA3245). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: Rat Testis tissue lysates,
    Lane 2: human JURKATwhole cell lysates,
    Lane 3: human HELA whole cell lysates,
    Lane 4: human MCF-7 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SLC16A4 antigen affinity purified polyclonal antibody (BA3245) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SLC16A4 at approximately 54 kDa. The expected band size for SLC16A4 is at 54 kDa.

    all(3)
  • IHC analysis of SLC16A4 using anti-SLC16A4 antibody (BA3245).
    SLC16A4 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SLC16A4 Antibody (BA3245) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(3)
  • IHC analysis of SLC16A4 using anti-SLC16A4 antibody (BA3245).
    SLC16A4 was detected in a paraffin-embedded section of human placenta cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SLC16A4 Antibody (BA3245) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(3)

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产品名称
Anti-SLC16A4 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
MCT 4; MCT 5; MCT4; MCT5; Monocarboxylate transporter 4; Monocarboxylate transporter 5; SLC16A4
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC
基因名称
SLC16A4
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the N-terminus of human SLC16A4, different from the related rat and mouse sequences by two amino acids.
计算分子量
54 kDa
实际分子量
54 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Monocarboxylate transporter 5, also called Solute carrier family 16 member 4 or MCT4, is a protein that in humans is encodes by the SLC16A4 gene. This gene is mapped to 1p13.3. It acts as the proton-linked monocarboxylate transporter. This gene catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.
Uniprot ID
O15374  
文献引用格式
SLC16A4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3245)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P).
基因名全称
solute carrier family 16, member 4(monocarboxylic acid transporter 5)
蛋白名全称
Monocarboxylate transporter 5
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-SLC16A4 Antibody

    筛选器: All WB IHC

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