Anti-PADI4 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

BA3341-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of PADI4 using anti-PADI4 antibody (BA3341-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: PANC whole cell lysates,
Lane 2: 293T whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PADI4 antigen affinity purified polyclonal antibody (BA3341-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PADI4 at approximately 68 kDa. The expected band size for PADI4 is at 74 kDa.
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产品简介>

产品名称

Anti-PADI4 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

HL 60 PAD; PAD; PAD4; PADI4; PADI5; PDI4; PDI5; Peptidylarginine deiminase IV

产品类型

Polyclonal

检验物种

human

应用范围

基因名称

PADI4

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human PADI4.

计算分子量

74 kDa

实际分子量

68 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

PADI4(Peptidylarginine Deiminase, Type IV), also known as PAD or PAD4, is a human protein which in humans is encoded by the PADI4 gene. Peptidylarginine deiminases, such as PADI4, make up a family of posttranslational modification enzymes that convert arginine residues to citrulline residues in the presence of calcium ion(Nakashima et al., 1999). Suzuki et al.(2003) found from sequence data that the PADI4 gene maps to 1p36. Cuthbert et al.(2004) described a process, deimination, that converts histone arginine to citrulline and antagonizes arginine methylation. They showed that PADI4 specifically deiminated arg2, arg8, arg17, and arg26 in the H3 tail. Deimination by PADI4 prevented arginine methylation by CARM1. Dimethylation of arginines prevented deimination by PADI4, although monomethylation still allowed deimination to take place. In vivo targeting experiments on an endogenous promoter demonstrated that PADI4 could repress hormone receptor-mediated gene induction. PADI4 was recruited to the pS2 promoter following hormone induction when the gene was transcriptionally downregulated, consistent with a repressive role for PADI4.

Uniprot ID

Q9UM07

文献引用格式

PADI4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3341-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

peptidyl arginine deiminase, type IV

蛋白名全称

Protein-arginine deiminase type-4

实验方案>

实验步骤

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