Western blot (WB): | 1:500-2000 |
Western blot analysis of GLUT1/SLC2A1 using anti-GLUT1/SLC2A1 antibody (BA3481-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: human SW620 whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GLUT1/SLC2A1 antigen affinity purified polyclonal antibody (BA3481-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GLUT1/SLC2A1 at approximately 55 kDa. The expected band size for GLUT1/SLC2A1 is at 54 kDa.
Western blot analysis of GLUT1/SLC2A1 using anti-GLUT1/SLC2A1 antibody (BA3481-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: human SW620 whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GLUT1/SLC2A1 antigen affinity purified polyclonal antibody (BA3481-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GLUT1/SLC2A1 at approximately 55 kDa. The expected band size for GLUT1/SLC2A1 is at 54 kDa.