Figure 1. Western blot analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: PC-3 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 Sigma/SFN antigen affinity purified polyclonal antibody (BA3752) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 Sigma/SFN at approximately 28 kDa. The expected band size for 14-3-3 Sigma/SFN is at 28 kDa.
Figure 2. IHC analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
14-3-3 Sigma/SFN was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
14-3-3 Sigma/SFN was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
14-3-3 Sigma/SFN was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IF analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
14-3-3 Sigma/SFN was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 6. IF analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
14-3-3 Sigma/SFN was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 7. Flow Cytometry analysis of A431 cells using anti-14-3-3 Sigma/SFN antibody (BA3752).
Overlay histogram showing A431 cells stained with BA3752 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
Figure 1. Western blot analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: PC-3 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 Sigma/SFN antigen affinity purified polyclonal antibody (BA3752) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 Sigma/SFN at approximately 28 kDa. The expected band size for 14-3-3 Sigma/SFN is at 28 kDa.
Figure 2. IHC analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
14-3-3 Sigma/SFN was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
14-3-3 Sigma/SFN was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
14-3-3 Sigma/SFN was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IF analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
14-3-3 Sigma/SFN was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 6. IF analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
14-3-3 Sigma/SFN was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 7. Flow Cytometry analysis of A431 cells using anti-14-3-3 Sigma/SFN antibody (BA3752).
Overlay histogram showing A431 cells stained with BA3752 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Figure 1. Western blot analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: PC-3 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 Sigma/SFN antigen affinity purified polyclonal antibody (BA3752) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 Sigma/SFN at approximately 28 kDa. The expected band size for 14-3-3 Sigma/SFN is at 28 kDa.
Figure 2. IHC analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
14-3-3 Sigma/SFN was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
14-3-3 Sigma/SFN was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
14-3-3 Sigma/SFN was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IF analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
14-3-3 Sigma/SFN was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 6. IF analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody (BA3752).
14-3-3 Sigma/SFN was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 7. Flow Cytometry analysis of A431 cells using anti-14-3-3 Sigma/SFN antibody (BA3752).
Overlay histogram showing A431 cells stained with BA3752 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 Sigma/SFN Antibody (BA3752) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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