Western blot analysis of Actin using anti-Actin antibody (BM0005).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysate,
Lane 2: rat PC-12 whole cell lysate,
Lane 3: mouse NIH3T3 cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Actin antigen affinity purified monoclonal antibody (Catalog # BM0005) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Actin at approximately 42KD. The expected band size for Actin is at 42KD.
IHC(F): Mouse Intestine Tissue
all(6) 
IHC(F): Mouse Spleen Tissue
all(6) 
IHC(F): Rat Intestine Tissue
all(6) 
IHC(F): Rat Spleen Tissue
all(6) 
IHC(F): Human Placenta Tissue
all(6) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of Actin using anti-Actin antibody (BM0005).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysate,
Lane 2: rat PC-12 whole cell lysate,
Lane 3: mouse NIH3T3 cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Actin antigen affinity purified monoclonal antibody (Catalog # BM0005) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Actin at approximately 42KD. The expected band size for Actin is at 42KD.
IHC(F): Mouse Intestine Tissue
IHC(F): Mouse Spleen Tissue
IHC(F): Rat Intestine Tissue
IHC(F): Rat Spleen Tissue
IHC(F): Human Placenta Tissue
Western blot analysis of Actin using anti-Actin antibody (BM0005).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysate,
Lane 2: rat PC-12 whole cell lysate,
Lane 3: mouse NIH3T3 cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Actin antigen affinity purified monoclonal antibody (Catalog # BM0005) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Actin at approximately 42KD. The expected band size for Actin is at 42KD.
IHC(F): Mouse Intestine Tissue
IHC(F): Mouse Spleen Tissue
IHC(F): Rat Intestine Tissue
IHC(F): Rat Spleen Tissue
IHC(F): Human Placenta Tissue
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