Anti-NF-κB p65/RELA Antibody (Clone#HH-18)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM3940

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-NF-κB p65/RELA antibody (BM3940). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SIHA whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NF-κB p65/RELA antigen affinity purified monoclonal antibody (BM3940) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NF-κB p65/RELA at approximately 70 kDa. The expected band size for NF-κB p65/RELA is at 65 kDa.
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Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder, using NF-κB p65 Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis of HT-1080 cells, using NF-κB p65 Antibody .
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产品简介>

产品名称

Anti-NF-κB p65/RELA Antibody (Clone#HH-18)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

NF Kappa B; NF κB; NFKB 3; NFKB p65; NFKB3; NF-κB; NF-κB3; p65; RELA; Transcription factor p65

产品类型

Monoclonal

检验物种

human, mouse

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

RELA

克隆号

HH-18

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human NF-B p65

计算分子量

60 kDa

实际分子量

70 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

RELA(V-REL AVIAN RETICULOENDOTHELIOSIS VIRAL ONCOGENE HOMOLOG A), also called NFKB3 or NFKB, p65 SUBUNIT. NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins, which inactivate NFKB by trapping it in the cytoplasm. The p65(RELA) heterodimer is the most abundant form of NFKB. And the RELA gene is located on 11q13.1. RELA is a nonhistone substrate of HDAC3 and that IKBA-dependent nuclear export of the HDAC3-deacetylated RELA replenishes the depleted cytoplasmic pool of latent NFKB-IKBA complexes for subsequent NFKB responses. RELA nucleocytoplasmic redistribution coincided with export of PPARG, and immunoprecipitation analysis indicated that PPARG-RELA association was dependent on the PPARG C-terminal ligand-binding domain. IKK-dependent phosphorylation of RELA on ser468 enhanced binding of GCN5 to RELA and RELA ubiquitination.

Uniprot ID

Q04206

文献引用格式

NF-κB p65/RELA Antibody (Clone#HH-18) (Boster Biological Technology, Wuhan, China. Catalog#BM3940)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Transcription factor p65

蛋白名全称

Transcription factor p65

实验方案>

实验步骤

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