Anti-NFkB/NFKB1 p105/p50 Antibody (Clone#ID-14)

Rabbit monoclonal antibody

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筛选器： All WB IHC

BM3946

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of NFkB/NFKB1 p105/p50 using anti-NFkB/NFKB1 p105/p50 antibody (BM3946). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat NRK whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NFkB/NFKB1 p105/p50 antigen affinity purified monoclonal antibody (BM3946) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NFkB/NFKB1 p105/p50 at approximately 105, 50 kDa. The expected band size for NFkB/NFKB1 p105/p50 is at 105, 50 kDa.
all(2)
Immunohistochemical analysis of paraffin-embedded human kidney, using NF-κB p105/p50 Antibody.
all(2)

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产品简介>

产品名称

Anti-NFkB/NFKB1 p105/p50 Antibody (Clone#ID-14)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

DNA binding factor KBF1; EBP 1; KBF1; NF kappa B; NFKB p105; NFKB p50; NFKB1; NFKB1; p105; NFKB1; p105; p50; nfkb1a; NF-κB; NF-κB 1; p105; p50

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

NFKB1

克隆号

ID-14

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human NFkB/NFKB1 (p105/p50)

计算分子量

105, 50 kDa

实际分子量

105, 50 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Nuclear factor NF-kappa-B p105 subunit, also called EBP-1 is a protein that in humans is encoded by the NFKB1 gene. By fluorescence in situ hybridization, the gene was assigned to human chromosome 4q24. NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing.

Uniprot ID

P19838

文献引用格式

NFkB/NFKB1 p105/p50 Antibody (Clone#ID-14) (Boster Biological Technology, Wuhan, China. Catalog#BM3946)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Nuclear factor NF-kappa-B p105 subunit

实验方案>

实验步骤

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