Anti-STAT1 Antibody (Clone#BEE-19)

Rabbit monoclonal antibody

说明书

筛选器： All WB IHC IP FCM

BM4110

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of anti-STAT1 antibody (BM4110). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STAT1 antigen affinity purified monoclonal antibody (BM4110) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for STAT1 at approximately 84, 87 kDa. The expected band size for STAT1 is at 87 kDa.
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Figure 2. IHC analysis of STAT1 using anti-STAT1 antibody (BM4110) .
STAT1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-STAT1 Antibody (BM4110) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 3. IHC analysis of STAT1 using anti-STAT1 antibody (BM4110) .
STAT1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-STAT1 Antibody (BM4110) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 4. IHC analysis of STAT1 using anti-STAT1 antibody (BM4110) .
STAT1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-STAT1 Antibody (BM4110) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-STAT1 Antibody (Clone#BEE-19)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ISGF 3; STAT1; STAT91

产品类型

Monoclonal

检验物种

human, mouse

应用范围

WB, IHC, IP, FCM

基因名称

STAT1

克隆号

BEE-19

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human STAT1

计算分子量

87 kDa

实际分子量

91 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Signal transducer and activator of transcription 1 (STAT1) is a transcription factor which in humans is encoded by the STAT1 gene. The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.

Uniprot ID

P42224

文献引用格式

STAT1 Antibody (Clone#BEE-19) (Boster Biological Technology, Wuhan, China. Catalog#BM4110)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

Signal transducer and activator of transcription 1-alpha/beta

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实验步骤

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