Anti-ERK1 (Phospho-T202/Y204)+ERK2 (Phospho-T185/Y187) Antibody (Clone#BIH-13)

Rabbit Monoclonal antibody

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BM4156

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of anti-ERK1 (Phospho-T202/Y204)+ERK2 (Phospho-T185/Y187) antibody (BM4156). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat skin tissue lysates,
Lane 6: mouse skin tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ERK1 (Phospho-T202/Y204)+ERK2 (Phospho-T185/Y187) antigen affinity purified monoclonal antibody (BM4156) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ERK1 (Phospho-T202/Y204)+ERK2 (Phospho-T185/Y187) at approximately 39 kDa. The expected band size for ERK1 (Phospho-T202/Y204)+ERK2 (Phospho-T185/Y187) is at 43 kDa.
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产品名称

Anti-ERK1 (Phospho-T202/Y204)+ERK2 (Phospho-T185/Y187) Antibody (Clone#BIH-13)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

MAP kinase 1; MAPK 1; ERT1; ERK-2; MAP kinase isoform p4; p42-MAPK; MAP kinase 2; MAPK 2; MAPK1; ERK2; PRKM1; PRKM2; ERK; ERK-2; MAPK2; NS13; P42MAPK; p38; p40; p41; p41mapk; MAP kinase 3; MAPK 3; ERT2; ERK-1; MAP kinase isoform p44; p44-MAPK; p44-ERK1; MAPK3; ERK1; PRKM3; HS44KDAP; HUMKER1A; P44ERK1; P44MAP; PRKM3

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IP

基因名称

MAPK1/MAPK3

克隆号

BIH-13

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Phospho-ERK1 (T202/Y204) + ERK2 (T185/Y187)

计算分子量

41 kDa/43 kDa

实际分子量

41 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Laminins are major proteins in the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. Laminins form independent networks and are associated with type IV collagen networks via entactin, fibronectin, and perlecan. They are important and biologically active parts of the basal lamina, influencing cell differentiation, migration, and adhesion, as well as phenotype and survival. Laminins are trimeric proteins that contain an α-chain, a β-chain, and a γ-chain, found in five, four, and three genetic variants, respectively. Laminins critically contribute to cell attachment and differentiation, cell shape and movement, maintenance of tissue phenotype, and promotion of tissue survival.

Uniprot ID

P27361/P28482

文献引用格式

ERK1 (Phospho-T202/Y204)+ERK2 (Phospho-T185/Y187) Antibody (Clone#BIH-13) (Boster Biological Technology, Wuhan, China. Catalog#BM4156)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Mitogen-activated protein kinase 3

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