Anti-a-SMA/ACTA2 Antibody (Clone#CAD-1)

Rabbit monoclonal antibody

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筛选器： All WB IHC IF ICC/IF FCM

BM4172

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-a-SMA/ACTA2 antibody (BM4172). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-a-SMA/ACTA2 antigen affinity purified monoclonal antibody (BM4172) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for a-SMA/ACTA2 at approximately 42 kDa. The expected band size for a-SMA/ACTA2 is at 42 kDa.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using alpha smooth muscle Actin Antibody.
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IF analysis of a-SMA/ACTA2 using anti-a-SMA/ACTA2 antibody (BM4172).
a-SMA/ACTA2 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-a-SMA/ACTA2 Antibody (BM4172) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(6)
IF analysis of a-SMA/ACTA2 using anti-a-SMA/ACTA2 antibody (BM4172).
a-SMA/ACTA2 was detected in a paraffin-embedded section of rat brain vessel tissue. The tissue section was incubated with rabbit anti-a-SMA/ACTA2 Antibody (BM4172) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(6)
Immunofluorescent analysis of A431 cells, using alpha smooth muscle Actin Antibody.
all(6)
all(6)

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产品简介>

产品名称

Anti-a-SMA/ACTA2 Antibody (Clone#CAD-1)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

a SMA; AAT6; ACTA2; ACTA2 Smooth muscle Actin; Actin, aortic smooth muscle, Actin; aortic smooth muscle Actin; ACTSA; ACTVS; Alpha actin 2; Alpha SMA; a-SMA; sma; smooth muscle actin; α SMA; α-sma

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF, ICC/IF, FCM

基因名称

ACTA2

克隆号

CAD-1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human alpha smooth muscle Actin

计算分子量

42 kDa

实际分子量

42 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Uniprot ID

P62736

Gene ID

文献引用格式

a-SMA/ACTA2 Antibody (Clone#CAD-1) (Boster Biological Technology, Wuhan, China. Catalog#BM4172)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

actin alpha 2, smooth muscle

蛋白名全称

ctin, aortic smooth muscle

实验方案>

实验步骤

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