Anti-ROCK1 Antibody (Clone#CDE-18)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM4203

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-ROCK1 antibody (BM4203). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ROCK1 antigen affinity purified monoclonal antibody (BM4203) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ROCK1 at approximately 170 kDa. The expected band size for ROCK1 is at 158 kDa.
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产品简介>

产品名称

Anti-ROCK1 Antibody (Clone#CDE-18)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

p160 ROCK 1; P160ROCK; PRO0435; ROCK1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

ROCK1

克隆号

CDE-18

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human ROCK1

计算分子量

158 kDa

实际分子量

158 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.

Uniprot ID

Q13464

文献引用格式

ROCK1 Antibody (Clone#CDE-18) (Boster Biological Technology, Wuhan, China. Catalog#BM4203)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Rho associated coiled-coil containing protein kinase 1

蛋白名全称

Rho-associated protein kinase 1

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实验步骤

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