Anti-BAD Antibody (Clone#CHC-2)

Rabbit monoclonal antibody

说明书

筛选器： All WB IHC ICC/IF IP FCM

BM4241

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti-BAD antibody (BM4241). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BAD antigen affinity purified monoclonal antibody (BM4241) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BAD at approximately 23 kDa. The expected band size for BAD is at 18 kDa.
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All lanes use the Antibody for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human colon cancer, using Bad Antibody.
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Immunofluorescent analysis of Hela cells, using Bad Antibody .
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-BAD Antibody (Clone#CHC-2)

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

BAD; BBC2; BBC6

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

BAD

克隆号

CHC-2

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Bad

计算分子量

18 kDa

实际分子量

23 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Uniprot ID

Q92934

文献引用格式

BAD Antibody (Clone#CHC-2) (Boster Biological Technology, Wuhan, China. Catalog#BM4241)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

Bcl2-associated agonist of cell death

实验方案>

实验步骤

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