Anti-YAP1 Antibody (Clone#CIE-25)

Rabbit monoclonal antibody

说明书

筛选器： All WB IHC ICC/IF IP FCM

BM4253

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of anti-YAP1 antibody (BM4253). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YAP1 antigen affinity purified monoclonal antibody (BM4253) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for YAP1 at approximately 70 kDa. The expected band size for YAP1 is at 54 kDa.
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Immunohistochemical analysis of paraffin-embedded human uterus, using YAP1 Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis of MCF7 cells, using YAP1 Antibody .
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-YAP1 Antibody (Clone#CIE-25)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

65 kDa Yes associated protein; YAP; YAP1; YAP2; YAP65; YKI; Yorkie homolog

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

YAP1

克隆号

CIE-25

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human YAP1

计算分子量

54 kDa

实际分子量

70 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

YAP1, also known as YAP or YAP65, is a potent oncogene, which is amplified in various human cancers. This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. It is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

Uniprot ID

P46937

文献引用格式

YAP1 Antibody (Clone#CIE-25) (Boster Biological Technology, Wuhan, China. Catalog#BM4253)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

Transcriptional coactivator YAP1

实验方案>

实验步骤

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