Anti-active+pro Caspase 3/CASP3(p17) Antibody(Clone#DGI-3)

Rabbit monoclonal antibody

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筛选器： All WB IHC IP

BM4340

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of anti-active + pro Caspase 3/CASP3 (p17) antibody (BM4340). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-active + pro Caspase 3/CASP3 (p17) antigen affinity purified monoclonal antibody (BM4340) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for active + pro Caspase 3/CASP3 (p17) at approximately 35 kDa,(cleaved)20/17/12 kDa. The expected band size for active + pro Caspase 3/CASP3 (p17) is at 32 kDa.
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Immunohistochemical analysis of paraffin-embedded human stomach, using active + pro Caspase 3 Antibody .
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Immunohistochemical analysis of paraffin-embedded Human melanoma, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded Human lung large cell cancer, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded Human colon, using the Antibody.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-active+pro Caspase 3/CASP3(p17) Antibody(Clone#DGI-3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Apopain; CASP 3; CASP3; Caspase 3; Caspase 3/p17; Caspase3; CPP 32; CPP32; CPP32B; Cysteine protease CPP32; Protein Yama; SCA 1; SREBP cleavage activity 1;

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IP

基因名称

CASP3

克隆号

DGI-3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human active + pro Caspase 3.

计算分子量

32 kDa

实际分子量

35 kDa,(cleaved)20/17/12 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Caspase 3 is a caspase protein which interacts with Survivin, XIAP, CFLAR, Caspase 8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located on 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. And the caspase-3 activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the caspase-3 influence on bone mineral density should be considered in any in vivo application of caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.

Uniprot ID

P42574

文献引用格式

active + pro Caspase 3/CASP3 (p17) Antibody (Clone#DGI-3) (Boster Biological Technology, Wuhan, China. Catalog#BM4340)

应用释义

WB-蛋白质免疫印迹法, IHC- 免疫组织化学法, ICC/IF-免疫细胞荧光, ICC-免疫细胞化学, IHC-F- 冰冻切片免疫组化, FCM-流式细胞术, ELISA-酶联免疫吸附测定, IP-免疫沉淀法 , IF-免疫组织荧光法, ChIP-染色质免疫沉淀法;

基因名全称

Caspase-3

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实验步骤

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