Anti-RHOA Antibody (Clone#FAC-18)

Rabbit monoclonal antibody

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筛选器： All WB ICC/IF FCM

BM4479

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-RHOA antibody (BM4479). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RHOA antigen affinity purified monoclonal antibody (BM4479) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RHOA at approximately 22 kDa. The expected band size for RHOA is at 22 kDa.
all(3)
Immunofluorescent analysis of Jurkat cells, using Rho A Antibody.
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all(3)

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产品简介>

产品名称

Anti-RHOA Antibody (Clone#FAC-18)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ARH12; ARHA; h12; Rho cDNA clone 12; RHO12; RHOA; RHOH12; Transforming protein RhoA

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF, FCM

基因名称

RHOA

克隆号

FAC-18

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Rho A

计算分子量

22 kDa

实际分子量

22 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

Uniprot ID

P61586

文献引用格式

RHOA Antibody (Clone#FAC-18) (Boster Biological Technology, Wuhan, China. Catalog#BM4479)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Transforming protein RhoA

实验方案>

实验步骤

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