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Anti-RAB7A Antibody (Clone#GHA-18)

Rabbit monoclonal antibody

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筛选器: All WB IHC ICC/IF FCM

BM4651

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti-RAB7A antibody (BM4651). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human A375 whole cell lysates,
    Lane 2: human HT1080 whole cell lysates,
    Lane 3: human U87 whole cell lysates,
    Lane 4: human HEL whole cell lysates,
    Lane 5: rat brain tissue lysates,
    Lane 6: rat L6 whole cell lysates,
    Lane 7: mouse brain tissue lysates,
    Lane 8: mouse C2C12 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB7A antigen affinity purified monoclonal antibody (BM4651) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB7A at approximately 23 kDa. The expected band size for RAB7A is at 23 kDa.

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产品名称
Anti-RAB7A Antibody (Clone#GHA-18)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
Endosome Marker; PRO2706; RAB7; RAB7A; Ras related protein Rab 7a
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF, FCM
基因名称
RAB7A
克隆号
GHA-18
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthesized peptide derived from human RAB7
计算分子量
23 kDa
实际分子量
23 kDa
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
浓度
500ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.
Uniprot ID
P51149  
文献引用格式
RAB7A Antibody (Clone#GHA-18) (Boster Biological Technology, Wuhan, China. Catalog#BM4651)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-200
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-200
Flow Cytometry (FCM):1:20

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    Anti-RAB7A Antibody (Clone#GHA-18)

    筛选器: All WB IHC ICC/IF FCM

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