Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-200 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
Flow Cytometry (FCM): | 1:20 |
Western blot analysis of anti-RAB7A antibody (BM4651). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A375 whole cell lysates,
Lane 2: human HT1080 whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat L6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB7A antigen affinity purified monoclonal antibody (BM4651) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB7A at approximately 23 kDa. The expected band size for RAB7A is at 23 kDa.
Western blot analysis of anti-RAB7A antibody (BM4651). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A375 whole cell lysates,
Lane 2: human HT1080 whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat L6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB7A antigen affinity purified monoclonal antibody (BM4651) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB7A at approximately 23 kDa. The expected band size for RAB7A is at 23 kDa.