Anti-mTOR (Phospho-S2448) Antibody (Clone#IFF-13)

Rabbit Monoclonal antibody

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筛选器： All WB IHC

BM4840

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-mTOR (Phospho-S2448) antibody (BM4840). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-mTOR (Phospho-S2448) antigen affinity purified monoclonal antibody (BM4840) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for mTOR (Phospho-S2448) at approximately 289 kDa. The expected band size for mTOR (Phospho-S2448) is at 289 kDa.
all(2)
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Phospho-mTOR (S2448) Antibody.
all(2)

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产品名称

Anti-mTOR (Phospho-S2448) Antibody (Clone#IFF-13)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

FLJ44809; FRAP; FRAP1; FRAP2; Mammalian target of rapamycin; MTOR; RAFT1; Rapamycin target protein 1; RAPT1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

MTOR

克隆号

IFF-13

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Phospho-mTOR (S2448)

计算分子量

289 kDa

实际分子量

289 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The mammalian target of rapamycin (mTOR), also known as the mechanistic target of rapamycin and FK506-binding protein 12-rapamycin-associated protein 1 (FRAP1), is a kinase that in humans is encoded by theMTOR gene. The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Uniprot ID

P42345

文献引用格式

mTOR (Phospho-S2448) Antibody (Clone#IFF-13) (Boster Biological Technology, Wuhan, China. Catalog#BM4840)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Serine/threonine-protein kinase mTOR

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实验步骤

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