Western blot (WB): | 1:500-2000 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
ImmunoPrecipitation (IP): | 1:20 |
Flow Cytometry (FCM): | 1:20 |
Western blot analysis of anti-PAI-1/SERPINE1 antibody (BM5169). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human A431 whole cell lysates,
Lane 6: human U-87MG whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PAI-1/SERPINE1 antigen affinity purified monoclonal antibody (BM5169) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PAI-1/SERPINE1 at approximately 45 kDa. The expected band size for PAI-1/SERPINE1 is at 45 kDa.
Western blot analysis of anti-PAI-1/SERPINE1 antibody (BM5169). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human A431 whole cell lysates,
Lane 6: human U-87MG whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PAI-1/SERPINE1 antigen affinity purified monoclonal antibody (BM5169) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PAI-1/SERPINE1 at approximately 45 kDa. The expected band size for PAI-1/SERPINE1 is at 45 kDa.