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Anti-PAI-1/SERPINE1 Antibody (Clone#ABHG-19)

Rabbit monoclonal antibody

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筛选器: All WB ICC/IF IP FCM

BM5169

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti-PAI-1/SERPINE1 antibody (BM5169). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human U251 whole cell lysates,
    Lane 2: human A549 whole cell lysates,
    Lane 3: human U2OS whole cell lysates,
    Lane 4: human HepG2 whole cell lysates,
    Lane 5: human A431 whole cell lysates,
    Lane 6: human U-87MG whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PAI-1/SERPINE1 antigen affinity purified monoclonal antibody (BM5169) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PAI-1/SERPINE1 at approximately 45 kDa. The expected band size for PAI-1/SERPINE1 is at 45 kDa.

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产品名称
Anti-PAI-1/SERPINE1 Antibody (Clone#ABHG-19)
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
PAI; PAI 1; PAI1; PAI-1; PLANH1; Serpin E1; SERPINE1; PAI-1
产品类型
Monoclonal
检验物种
human
应用范围
WB, ICC/IF, IP, FCM
基因名称
SERPINE1
克隆号
ABHG-19
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthesized peptide derived from human PAI1
计算分子量
45 kDa
实际分子量
45 kDa
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Plasminogen activator inhibitor-1 (PAI-1) also known as endothelial plasminogen activator inhibitoror serpin E1 is a protein that in humans is encoded by the SERPINE1 gene. This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Uniprot ID
P05121  
文献引用格式
PAI-1/SERPINE1 Antibody (Clone#ABHG-19) (Boster Biological Technology, Wuhan, China. Catalog#BM5169)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
Plasminogen activator inhibitor 1
推荐稀释比
Western blot (WB):1:500-2000
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-200
ImmunoPrecipitation (IP):1:20
Flow Cytometry (FCM):1:20

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    Anti-PAI-1/SERPINE1 Antibody (Clone#ABHG-19)

    筛选器: All WB ICC/IF IP FCM

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