Western blot (WB): | 1:500-2000 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
ImmunoPrecipitation (IP): | 1:50 |
Western blot analysis of anti-xCT/SLC7A11 antibody (BM5318). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-xCT/SLC7A11 antigen affinity purified monoclonal antibody (BM5318) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for xCT/SLC7A11 at approximately 55 kDa. The expected band size for xCT/SLC7A11 is at 55 kDa.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis using the Antibody.
Western blot analysis of anti-xCT/SLC7A11 antibody (BM5318). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-xCT/SLC7A11 antigen affinity purified monoclonal antibody (BM5318) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for xCT/SLC7A11 at approximately 55 kDa. The expected band size for xCT/SLC7A11 is at 55 kDa.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis using the Antibody.