Anti-ATG7 Antibody (Clone#ACHA-1)

Rabbit monoclonal antibody

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筛选器： All WB ICC/IF

BM5322

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-ATG7 antibody (BM5322). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human SiHa whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATG7 antigen affinity purified monoclonal antibody (BM5322) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATG7 at approximately 78 kDa. The expected band size for ATG7 is at 78 kDa.
all(3)
Western blot analysis of anti-ATG7 antibody (BM5322). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela- WT whole cell lysates,
Lane 2: human Hela-ATG7 KO whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATG7 antigen affinity purified monoclonal antibody (BM5322) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATG7 at approximately 78 kDa. The expected band size for ATG7 is at 78 kDa.
all(3)
Immunofluorescent analysis using the Antibody.
all(3)

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产品简介>

产品名称

Anti-ATG7 Antibody (Clone#ACHA-1)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

APG7 LIKE; APG7L; ATG7; Autophagy related protein 7; GSA7; hAGP7

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF

基因名称

ATG7

克隆号

ACHA-1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Atg7 (Apg7) Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.

计算分子量

78 kDa

实际分子量

78 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Autophagy related 7 is a protein in humans encoded by ATG7 gene. It is mapped to 3p25.3. This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants.

Uniprot ID

O95352

文献引用格式

ATG7 Antibody (Clone#ACHA-1) (Boster Biological Technology, Wuhan, China. Catalog#BM5322)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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