Anti-HPRT1 Antibody (Clone#ADAB-8)

Rabbit monoclonal antibody

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筛选器： All WB IHC IP

BM5361

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-HPRT1 antibody (BM5361). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549- WT whole cell lysates,
Lane 2: human A549-HPRT1 KO whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HPRT1 antigen affinity purified monoclonal antibody (BM5361) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HPRT1 at approximately 25 kDa. The expected band size for HPRT1 is at 25 kDa.
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Western blot analysis of HPRT expression in A549 cell lysate.
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All lanes use the Antibody for 1 hour at room temperature.
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All lanes use the Antibody for 1 hour at room temperature.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-HPRT1 Antibody (Clone#ADAB-8)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

HGPRT; HGPRTase; HPRT; HPRT1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IP

基因名称

HPRT1

克隆号

ADAB-8

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human HPRT

计算分子量

25 kDa

实际分子量

25 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) is an enzyme encoded in humans by the HPRT1 gene. The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Uniprot ID

P00492

文献引用格式

HPRT1 Antibody (Clone#ADAB-8) (Boster Biological Technology, Wuhan, China. Catalog#BM5361)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

实验步骤

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