Immunohistochemistry (IHC): | 1:50-400 |
Immunofluorescence (IF): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Figure 1. IF analysis of cdhr1a using anti-cdhr1a antibody (DZ07988).
cdhr1a was detected in a paraffin-embedded section of Figure 1. IF analysis of Cdhr1a using anti-Cdhr1a antibody (DZ07988).
Cdhr1a was detected in paraffin-embedded section of five-day-old zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Cdhr1a Antibody (DZ07988) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight?488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used. tissue. The tissue section was incubated with rabbit anti-cdhr1a Antibody (DZ07988) at a dilution of 1:100. Biotin conjugated goat anti-rabbit IgG (Catalog # BA1003) was used as secondary antibody. The tissue section was developed using DyLight 488 Conjugated Avidin (Catalog # BA1128).
Figure 2. IHC analysis of cdhr1a using anti-cdhr1a antibody (DZ07988) .
cdhr1a was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-cdhr1a Antibody (DZ07988) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of cdhr1a using anti-cdhr1a antibody (DZ07988) .
cdhr1a was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-cdhr1a Antibody (DZ07988) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 1. IF analysis of cdhr1a using anti-cdhr1a antibody (DZ07988).
cdhr1a was detected in a paraffin-embedded section of Figure 1. IF analysis of Cdhr1a using anti-Cdhr1a antibody (DZ07988).
Cdhr1a was detected in paraffin-embedded section of five-day-old zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Cdhr1a Antibody (DZ07988) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight?488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used. tissue. The tissue section was incubated with rabbit anti-cdhr1a Antibody (DZ07988) at a dilution of 1:100. Biotin conjugated goat anti-rabbit IgG (Catalog # BA1003) was used as secondary antibody. The tissue section was developed using DyLight 488 Conjugated Avidin (Catalog # BA1128).
Figure 2. IHC analysis of cdhr1a using anti-cdhr1a antibody (DZ07988) .
cdhr1a was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-cdhr1a Antibody (DZ07988) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of cdhr1a using anti-cdhr1a antibody (DZ07988) .
cdhr1a was detected in a paraffin-embedded section of zebrafish kidney tissue. The tissue section was incubated with rabbit anti-cdhr1a Antibody (DZ07988) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.