Figure 1. Western blot analysis of Casc3 using anti-Casc3 antibody (DZ41294). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Marker,
Lane 2: Zebrafish,
Lane 3: Zebrafish head tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Casc3 antigen affinity purified polyclonal antibody (DZ41294) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Casc3 at approximately 76 kDa. The expected band size for Casc3 is at 76 kDa.
Figure 2. IHC analysis of Casc3 using anti-Casc3 antibody (DZ41294) .
Casc3 was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-Casc3 Antibody (DZ41294) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of Casc3 using anti-Casc3 antibody (DZ41294). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Marker,
Lane 2: Zebrafish,
Lane 3: Zebrafish head tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Casc3 antigen affinity purified polyclonal antibody (DZ41294) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Casc3 at approximately 76 kDa. The expected band size for Casc3 is at 76 kDa.
Figure 2. IHC analysis of Casc3 using anti-Casc3 antibody (DZ41294) .
Casc3 was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-Casc3 Antibody (DZ41294) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 1. Western blot analysis of Casc3 using anti-Casc3 antibody (DZ41294). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Marker,
Lane 2: Zebrafish,
Lane 3: Zebrafish head tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Casc3 antigen affinity purified polyclonal antibody (DZ41294) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Casc3 at approximately 76 kDa. The expected band size for Casc3 is at 76 kDa.
Figure 2. IHC analysis of Casc3 using anti-Casc3 antibody (DZ41294) .
Casc3 was detected in a paraffin-embedded section of zebrafish brain tissue. The tissue section was incubated with rabbit anti-Casc3 Antibody (DZ41294) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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