Rat BDNF ELISA Kit

96T/1880

Abrineurin; BDNF

Expression system for standard: NS0; Immunogen sequence: H129-R247

There is no detectable cross-reactivity with other relevant proteins.

31.2pg/ml-2000pg/ml

BDNF

brain-derived neurotrophic factor

P23363  

Rat BDNF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0308)

Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury.

Catalog number	Description	Quantity
EK0308-CAP	96-well plate precoated with anti- rat BDNF antibody	1
EK0308-ST	Lyophilized recombinant rat BDNF standard	10ng/tube×2
EK0308-DA	Biotinylated anti- rat BDNF antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	88	292	906	84	273	977
Standard deviation	4.4	16.06	70.66	4.2	19.11	77.18
CV(%)	5%	5.5%	7.8%	5%	7%	7.9%

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.016	0.051	0.082	0.161	0.337	0.834	1.668	2.444

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

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Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA, citrate)