指标别名
ciliary neurotrophic factor; CNTF; HCNTF
免疫原
Expression system for standard: E.coli; Immunogen sequence: A2-M200
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
ciliary neurotrophic factor
文献引用格式
Rat CNTF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0324)
背景资料
Ciliary neurotrophic factor (CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The hCNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid (aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85% homology with the deduced aa sequence encoding hCNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.
试剂盒内容
| Catalog number | Description | Quantity |
| EK0324-CAP | 96-well plate precoated with anti- rat CNTF antibody | 1 |
| EK0324-ST | Lyophilized recombinant rat CNTF standard | 10ng/tube×2 |
| EK0324-DA | Biotinylated anti- rat CNTF antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 82 | 276 | 868 | 80 | 293 | 809 |
| Standard deviation | 4.59 | 16.83 | 60.76 | 5.52 | 23.14 | 60.67 |
| CV(%) | 5.6% | 6.1% | 7% | 6.9% | 7.9% | 7.5% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.002 | 0.063 | 0.126 | 0.238 | 0.442 | 0.838 | 1.464 | 2.035 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)