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Rat CNTF ELISA Kit

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EK0324

  • 96T ¥2280
  • 货期: 现货
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产品名称
Rat CNTF ELISA Kit
规格/价格
96T|2280
指标别名
ciliary neurotrophic factor; CNTF; HCNTF
免疫原
Expression system for standard: E.coli; Immunogen sequence: A2-M200
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2pg/ml-2000pg/ml
基因名称
CNTF
基因名全称
ciliary neurotrophic factor
Uniprot ID
P20294  
文献引用格式
Rat CNTF ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0324)
背景资料
Ciliary neurotrophic factor (CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The hCNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid (aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85% homology with the deduced aa sequence encoding hCNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.
试剂盒内容
Catalog numberDescriptionQuantity
EK0324-CAP96-well plate precoated with anti- rat CNTF antibody1
EK0324-STLyophilized recombinant rat CNTF standard10ng/tube×2
EK0324-DABiotinylated anti- rat CNTF antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Sample123123
n161616242424
Mean(pg/ml)8227686880293809
Standard deviation4.5916.8360.765.5223.1460.67
CV(%)5.6%6.1%7%6.9%7.9%7.5%
典型数据示例
Concentration (pg/ml)031.262.512525050010002000
O.D.0.0020.0630.1260.2380.4420.8381.4642.035
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
检验物种
rat
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)

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