Human Fibronectin/FN1 ELISA Kit

96T/2280

CIG; Cold insoluble globulin; ED B; Fibronectin; fibronectin 1; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF

from plasma

There is no detectable cross-reactivity with other relevant proteins.

156 pg/ml - 10000 pg/ml

FN1

fibronectin 1

P02751  

Human Fibronectin/FN1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0349)

Fibronectin(FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer. The standard used in this kit is isolated from human plasma with the molecular mass of 200-250KDa.

25-30分钟

Catalog number	Description	Quantity
EK0349-CAP	96-well plate precoated with anti- human Fibronectin antibody	1
EK0349-ST	Lyophilized human Fibronectin standard	10ng/tube×2
EK0349-DA	Biotinylated anti- human Fibronectin antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	312	1118	5412	341	1105	4997
Standard deviation	14.35	65.96	308.48	19.43	76.24	349.79
CV(%)	4.6	5.9	5.7	5.7	6.9	7

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.037	0.108	0.190	0.328	0.527	1.003	1.418	1.983

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)

3.5h