产品名称
Human IFN Gamma/IFNG ELISA KIt
指标别名
IFG; IFI; IFN gamma; IFN γ; IFNG; IFN-gamma; IFN-γ
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
文献引用格式
Human IFN Gamma/IFNG ELISA KIt (Boster Biological Technology, Wuhan, China. Catalog#EK0373)
背景资料
Interferon-gamma(IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer(NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0373-CAP | 96-well plate precoated with anti- human IFN gamma antibody | 1 |
EK0373-ST | Lyophilized recombinant human IFN gamma standard | 10ng/tube×2 |
EK0373-DA | Biotinylated anti- human IFN gamma antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 34 | 150 | 469 | 33 | 139 | 437 |
Standard deviation | 1.73 | 8.7 | 29.54 | 1.91 | 9.73 | 31.9 |
CV(%) | 5.1 | 5.8 | 6.3 | 5.8 | 7 | 7.3 |
典型数据示例
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.011 | 0.056 | 0.098 | 0.183 | 0.366 | 0.646 | 1.382 | 2.405 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum