产品名称
Rat IL-1 beta/IL1B ELISA Kit
指标别名
IL1 beta; Catabolin; IL 1; IL 1 beta; IL1 BETA; IL-1 beta; IL1- beta; IL1B; IL1beta; IL1F2; IL-1β; Interleukin 1 beta; interleukin 1; beta; Pro IL-1β; IL 1B; IL-1B
免疫原
Expression system for standard: E.coli; Immunogen sequence: V117-S268
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
interleukin 1, beta
文献引用格式
Rat IL-1 beta/IL1B ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0393)
背景资料
The Interleukin 1 family is a group of 11 beta trefoil cytokines. Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response . Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta belongs to proinflammatory cytokines, promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1 (VCAM-1) on hepatic sinusoidal endothelium (HSE).
试剂盒内容
Catalog number | Description | Quantity |
EK0393-CAP | 96-well plate precoated with anti- rat IL-1 beta antibody | 1 |
EK0393-ST | Lyophilized recombinant rat IL-1 beta standard | 10ng/tubex2 |
EK0393-DA | Biotinylated anti- rat IL-1 beta antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 58 | 233 | 962 | 62 | 241 | 881 |
Standard deviation | 3.65 | 11.41 | 69.26 | 4.21 | 12.29 | 68.71 |
CV(%) | 6.3% | 4.9% | 7.2% | 6.8% | 5.1% | 7.8% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.022 | 0.072 | 0.136 | 0.213 | 0.364 | 0.779 | 1.626 | 2.440 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)