产品名称
Human IL11 ELISA Kit
指标别名
Adipogenesis inhibitory factor; AGIF; IL 11; IL11; IL-11; interleukin 11; Oprelvekin
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
文献引用格式
Human IL11 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0419)
背景资料
Interleukin 11(IL-11) is a protein that in humans is encoded by the IL11 gene. The protein encoded by this gene is a member of the gp130 family of cytokines. This gene is mapped to 19q13.42. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL-11 has been demonstrated to improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. What's more, IL-11 can stimulate the growth of certain lymphocytes and it has functions in many other tissues, including the brain, gut, testis and bone. In transgenic mice, overexpression of the human IL11 gene resulted in the stimulation of bone formation to increase cortical thickness and strength of long bones.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0419-CAP | 96-well plate precoated with anti- human IL-11 antibody | 1 |
EK0419-ST | Lyophilized recombinant human IL-11 standard | 10ng/tubex2 |
EK0419-DA | Biotinylated anti- human IL-11 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 70 | 290 | 882 | 67 | 281 | 907 |
Standard deviation | 3.22 | 20.3 | 42.33 | 3.81 | 23.04 | 45.35 |
CV(%) | 4.6 | 7 | 4.8 | 5.7 | 8.2 | 5 |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.082 | 0.125 | 0.167 | 0.249 | 0.451 | 0.784 | 1.362 | 2.212 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)