产品名称
Human Laminin ELISA Kit
指标别名
Lamb2; LAMB2_HUMAN; LAMC1; LAMC2; LAMC3; laminin A; Laminin B; Laminin B1s chain; Laminin gamma 1; Laminin gamma 2; Laminin gamma 3; Laminin subunit beta-2; Laminin-11 subunit beta; Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-3 subunit beta; Laminin-4 subunit beta; Laminin-7 subunit beta; Laminin-9 subunit beta; OCCM; S-LAM beta; S-laminin subunit beta; LAMA1; LAMA2; LAMB1; LAMB2
免疫原
from human fibroblasts
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名称
LAMA1/A2/A3/A4/A5/B1/B2/B3/B4/C1/C2/C3
基因名全称
laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, beta 2(laminin S)
文献引用格式
Human Laminin ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0434)
背景资料
Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide.2 The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0434-CAP | 96-well plate precoated with anti- human LAMA1 antibody | 1 |
| EK0434-ST | Lyophilized human LAMA1 standard | 10ng/tubex2 |
| EK0434-DA | Biotinylated anti- human LAMA1 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 318 | 1051 | 4462 | 295 | 1015 | 4289 |
| Standard deviation | 23.21 | 47.29 | 348.03 | 26.25 | 58.87 | 411.74 |
| CV(%) | 7.3 | 4.5 | 7.8 | 8.9 | 5.8 | 9.6 |
典型数据示例
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.146 | 0.180 | 0.207 | 0.279 | 0.382 | 0.750 | 1.187 | 2.224 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates and Serum
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