产品名称
Human MCP-3/CCL7 ELISA Kit
指标别名
C C motif chemokine 7; CCL7; chemokine (C C motif) ligand 7; FIC; MARC; MCP 3; MCP3; Monocyte chemotactic protein 3; NC28; SCYA6; SCYA7; Small inducible cytokine A7
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名全称
chemokine (C-C motif) ligand 7
文献引用格式
Human MCP-3/CCL7 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0443)
背景资料
Chemokine(C-C motif) ligand 7(CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3(MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0443-CAP | 96-well plate precoated with anti- human CCL7 antibody | 1 |
| EK0443-ST | Lyophilized recombinant human CCL7 standard | 10ng/tubex2 |
| EK0443-DA | Biotinylated anti- human CCL7 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 38 | 169 | 505 | 37 | 158 | 504 |
| Standard deviation | 2.39 | 12.67 | 27.27 | 2.55 | 12.79 | 31.75 |
| CV(%) | 6.3 | 7.5 | 5.4 | 6.9 | 8.1 | 6.3 |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.013 | 0.061 | 0.105 | 0.201 | 0.368 | 0.751 | 1.474 | 2.256 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)