Human MMP7 ELISA Kit

96T/2480

Matrilysin; Matrin; Matrix metalloproteinase 7; MMP 7; MMP7; MPSL1; PUMP 1; Pump 1 protease; PUMP1; Uterine metalloproteinase

NSO, L18-K267

There is no detectable cross-reactivity with other relevant proteins.

156 pg/ml - 10000 pg/ml

MMP7

matrix metallopeptidase 7 (matrilysin, uterine)

P09237  

Human MMP7 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0463)

Matrix metalloproteinase-7(MMP-7) previously called putative metalloproteinase I(PUMP1) or matrilysin. The PUMP1 gene has been identified through studies of collagenase-related connective-tissue-degrading metalloproteinases produced by human tumors. The PUMP I protein has 267 amino acids and is significantly shorter than stromelysin or collagenase(477 and 469 amino acids, respectively). Matrix metalloproteinases play a crucial role in tumor invasion and metastasis. Matrilysin, a member of the matrix metalloproteinase family, is structurally different from the other matrix metalloproteinases by virtue of the absence of a conserved COOH-terminal protein domain. In addition, matrilysin mRNA is regulated in a specific and distinct manner in normal and malignant tissues. Matrilysin has been shown to correlate with nodal or distant metastasis in colorectal carcinomas; however, its implication in early invasive colorectal carcinomas has not been determined.1 Matrilysin is also a mediator of pulmonary fibrosis and a potential therapeutic target. The standard product used in this kit is recombinant human MMP-7, consisting of 250 amino acids with the molecular mass of 28KDa. The detected MMP-7 includes zymogen and active enzyme.

20-25分钟

Catalog number	Description	Quantity
EK0463-CAP	96-well plate precoated with anti- human MMP-7 antibody	1
EK0463-ST	Lyophilized recombinant human MMP-7 standard	10ng/tubex2
EK0463-DA	Biotinylated anti- human MMP-7 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	272	1042	5090	256	1008	5032
Standard deviation	12.51	43.76	335.94	15.36	47.37	412.62
CV(%)	4.6	4.2	6.6	6	4.7	8.2

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.039	0.083	0.144	0.202	0.436	0.867	1.573	2.192

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (heparin), Saliva and Urine

3.5h